|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 7. Tangential migration from the septum is severely impaired in
Vax1-/- embryos. (A,B) Nissl staining of coronal sections
from wild-type and mutant brains at E16.5. There is complete loss of the
septum in Vax1-/- telencephalon. (C,F) (Top) The
experimental procedure. The red spots represent DiI crystals placed at E13.5
in an organotypic slice culture assay. (Bottom) Bright-field images of
wild-type (C) and mutant (F) slices corresponding to those shown in the
scheme. Sections are taken at comparable rostrocaudal levels. (D,E,G,H)
Fluorescent images of the same slices from wild-type (D,E) and
Vax1-/- (G,H) embryos; (E,H) high-magnification
photographs of the region boxed in D and G, respectively. Cells that migrate
along a lateral-tangential pathway are present in the wild-type slice (E,
white arrows) but there is complete loss of this stream in the mutant brain
(H). lv, lateral ventricle; lge, lateral ganglionic eminence; sa, septal area;
sa*, presumptive septal area.
|
