DEV01289 Supplementary Material

Files in this Data Supplement:

• Supplemental Table (PDF)
• Supplemental Figure 1 (Eve)
• Supplemental Figure 1 (Hairy) -

  Fig. S1. Alignments of Eve, Hairy and Wg homologues. Deduced amino acid sequences of Eve, Hairy and Wg from Drosophila melanogaster (Dme), Episyrphus balteatus (Eba), Megaselia abdita (Mab), Platypeza consobrina (Pco), Empis livida (Eli), Haematopota pluvialis (Hpl), Coboldia fuscipes (Cfu), Anopheles gambiae (Aga) and Clogmia albipunctata (Cal) were aligned using the Clustal method of Megalign/DNAstar software. Identical positions are shaded in yellow; dots indicate gaps in the known sequence. The homeodomain (HD), helix-loop-helix region (HLH) and orange domain are boxed. Eve alignment starts at HD (position 70 of Dme-Eve), Hairy and Wg alignments start 3¢ to an intron in the Drosophila sequence (position 66 of Dme-Hairy, position 367 of Dme-Wg). Start of the corresponding DNA sequences used to generate RNA for injection is printed in red (unless laying outside the alignment). The blue arrows indicate the starting position of the sequence used for tree construction (Fig. 1). Accession numbers: Dme-Eve SWP, P06602, Dme-Hairy SPTREMBL, Q95NU9; Dme-Wg SPTREMBL, Q8MQP9.

• Supplemental Figure 2 -

  Fig. S2. Episyrphus blastoderm cytoarchitecture. Three successive stages of Episyrphus balteatus blastoderm embryos are shown from left to right. The embryos were staged according to the expression pattern of h, which evolves similar to the h pattern in Drosophila (darker regions are h mRNA expression domains). The nuclei move from an apical to a central position within the peripheral blastoderm as embryogenesis progresses. White arrowheads mark the transition from peripheral cytoplasm to yolk.