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Fig. 2. XNIF encodes for a vegetally localized RNA in Xenopus oocytes.
Whole-mount in-situ hybridization was carried out using albino oocytes of
different stages. For a detailed analysis, sections of stained stage I and
stage II oocytes were prepared. (A) In stage I oocytes, XNIF RNA is enriched
in the mitochondrial cloud (examples marked by arrows). The cell nucleus is
indicated by a dashed line in one oocyte. (B) In stage II oocytes, XNIF
accumulates at the tip of the vegetal cortex (red arrow). In stage III-IV
oocytes, XNIF RNA is associated with the vegetal cortex (black arrow). (C)
Vegetal-cortical localization of XNIF in stage V/VI oocytes. (D) Section of
whole-mount in-situ stained stage I oocyte. XNIF transcript is enriched in the
mitochondrial cloud adjacent to the germinal vesicle. (E) Section of
whole-mount in-situ stained stage II oocyte. XNIF is associated with reticular
structures in a wedge-shaped region beneath the germinal vesicle. Scale bar:
100 µm in (A,D,E) and 1 mm in (B,C). (F) XNIF expression in a stage 33
Xenopus albino embryo. Whole-mount in-situ hybridization staining
with a XNIF specific antisense probe is shown.
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