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Fig. 3. The 5'-UTR of XNIF contains a vegetal localization element.
lacZ-tagged RNAs were injected into stage II-III albino oocytes and
visualized by whole-mount in-situ hybridization after culturing in
vitellogenin-enriched L15 medium. Vegetal localization was observed in oocytes
injected with transcripts containing the 5'-UTR (A) and with fragments
containing nucleotides 252-551 (C) and 252-381 (D), but not with the coding
region or 3'-UTR (B). Scale bar: 
500 µm. The section in (E)
shows the wedge and cortical staining of a stage II oocyte that has been
injected with a transcript containing the nucleotides 252-551 as shown in (C).
(F) Schematic representation of the constructs used for XNIF localization
element mapping experiments. 5'-UTR, ORF and 3'-UTR are
represented by yellow, blue and red, respectively. Corresponding nucleotide
positions of the XNIF cDNA are indicated. Capability of vegetal localization
is marked with (+), absence of vegetal localization by (-). A weak and not
always reproducible localizing activity was also observed for the fragment nt
1002-1308 and is marked by an asterisk.
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