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Fig. 4. XNIF-LE mediates accumulation in the mitochondrial cloud.
lacZ-tagged RNAs were injected into stage I-III oocytes and
localization was visualized by whole-mount in-situ hybridization. Localization
to the vegetal cortex in stage III and enrichment in the mitochondrial cloud
in stage I was observed with the transcript nt 252-551 (A,F). The transcript
nt 252-381 does not accumulate in the mitochondrial cloud, but is transported
in stage III oocytes (B,G). A transcript containing nucleotides 415-551
neither localizes in stage I nor in stage III oocytes (C,H). A transcript
containing a duplication of nucleotides 252-381 in tandem repeat localizes to
the mitochondrial cloud in stage I and to the vegetal cortex in stage II-III
oocytes (D,I). No localization to the mitochondrial cloud or vegetal cortex
was observed with a transcript containing the XNIF 3'-UTR (E,J). Scale
bar: 
100 µm in A-D; 500 µm in F-J. (K) The nucleotide sequence
of the nt 252-551 localization element is shown. CAC-sequence element
containing motifs and isolated CAC triplets are shaded. The nt 252-381 minimal
sequence that mediates localization in stage III oocytes is boxed.
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