spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 6. The XNIF localization element interacts with Vg1RBP and Prrp in co-immunoprecipitation experiments. lacZ-RNA, XNIF-LE (nt 252-551) as well as XNIF nt 252-381, XNIF nt252-381 in a tandem repeat (nt 252-381-tr) and Vg1-LE are radioactively labeled by in-vitro transcription. Vg1RBP, Prrp, VgRBP71 and L5 were produced by coupled transcription and translation in the TNT reticulocyte lysate system and incubated with radiolabeled RNAs. RNPs were immunoprecipitated by the myc-epitope and unbound RNA from the supernatant (sup.) and coprecipitated (bound) RNAs were analyzed by denaturing PAGE. Different labeled RNAs are marked on the left-hand side; in-vitro translated proteins are indicated on the top panel.





Right arrow Return to article