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Fig. 1. ERK activation in the neural tube. (A) Whole-mount in-situ hybridization for Fgf8 at the 8-somite stage (HH9+). (B-E) Distribution of activated ERK revealed immunohistochemically with anti-dpERK antibody in normal embryos. (B) Eight-somite stage (HH9+). (C) Ten-somite stage (HH10). (D) Twelve-somite stage (HH11-). (E) Fourteen-somite stage (HH11+). ERK is activated around the isthmus (arrow on B) and the anterior tip of the neural tube at the 8-somite stage. By the 14-somite stage, ERK has been inactivated in the metencephalon.





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