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Fig. 2. ERK activation by Fgf8 signal. (A) Immunohistochemistry with anti-dpERK 1
hour after insertion of an SU5402-soaked bead (asterisk). ERK activation is
repressed by SU5402, an inhibitor of the Fgf receptor. (B) Dorsal and (C)
lateral view of an E14.5 brain after misexpression of Fgf8b. Instead
of the tectum, cerebellum has differentiated in the mesencephalic region
(arrow). (D) Dorsal view of an E6.5 brain after misexpression of
Fgf8a. The tectum enlarged because the fate of the diencephalic alar
plate was changed to tectum. (E) Misexpression of GFP at 3 hours after
electroporation. The GFP misexpression site corresponds to that of Fgf8 shown
in (F-H). (F-H) Immunohistochemistry with anti-dpERK antibody after
misexpression of Fgf8a 1 µg/µl (F), Fgf8b 1
µg/µl (G), Fgf8b 0.01 µg/µl (H). ERK was activated only
in the diencephalon by Fgf8a (F). ERK was activated at the site where
Fgf8b was misexpressed through the diencephalon, mesencephalon and
metencephalon (G). Misexpression of Fgf8b at a concentration of 0.01
µg/µl caused ERK activation only in the diencephalon (H), as was the
case of Fgf8a misexpression. di, diencephalon; mes, mesencephalon;
met, metencephalon; tel, telencephalon; tect, tectum; cer, cerebellum;
cer-ect, ectopic cerebellum. Scale bars: 200 µm (A,F-H), 4 mm (B-D).
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