QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 4. Effects of Ras^S17N misexpression on Otx2, Gbx2 and Fgf8. Effects on Otx2 (A-C,J,K), Gbx2 (D-F,L-N) and Fgf8 (G-I,OQ). Misexpression site of Ras^S17N (brown in C,F,I) is assessed by immunohistochemistry against HA-tag. Otx2, Gbx2 and Fgf8 expression is represented as blue by in-situ hybridization. Twenty-four and 42 hours after electroporation (A-I and J-Q, respectively). Left (control) side of the brain vesicles (A,D,G,L) and right (transfected) side (B,C,E,F,H,I,M,P). Dorsal view (J,O). Higher magnification (B',C',E',F',H',I',K,N,Q). The rectangular area on (E) corresponds to (E'). By 24 hours after electroporation, Otx2 was induced in the metencephalon by Ras^S17N misexpression (B,C,B',C'). Gbx2 was repressed in the metencephalon by Ras^S17N misexpression (E,F,E',F'). Endogenous Fgf8 expression was also repressed, but was induced in the periphery of Ras^S17N expression in the metencephalon (H,I,H',I'). By 42 hours after electroporation, the boundary of patchy expression of Otx2 in the metencephalic region became blurred (J,K), and Otx2 expression area expanded (arrowheads in K). Repression of Gbx2 and Fgf8 just posterior to the proper mesencephalon became wider, leaving a Gbx2- and Fgf8-free region (L-Q). di, diencephalon; mes, mesencephalon; met, metencephalon; cont, control side; exp, experimental side. Scale bars: 200 µm (AC,D-F,G-I,J,L,M,O,P), 100 µm (B',C',E',F',H',I',K,N,Q).

Return to article