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Fig. 2. Neural stem cells can generate skeletal and cardiac myocytes. In order to
demonstrate the potential of NSCs, CD31- CD35-
EGFP+ cortical progenitor cells were expanded for 6 days in
MB-media prior to plating on an adherent surface to complete differentiation.
By both reverse transcription polymerase chain reaction (RT-PCR) and
immunohistochemical analyses, differentiated progeny expressed markers of
myogenic differentiation. (A) RT-PCR was performed to assess the steady state
levels of MyoD expression of skeletal muscle actin and cardiac actin mRNAs at
7 and 18 days of MLNSC differentiation. A photomicrograph of an ethidium
bromide stained agarose gel displaying the amplification products for
reactions using mRNA isolated from cultures at day 7 and day 18 of
differentiation is shown. (B-D) Time course of sk-MHC immunoreactivity within
differentiating clones 9 days (B), 11 days (C) and 18 days (D) post-plating is
presented. (E) Immunofluorescent images for cardiac-specific MHC and the gap
junction protein, Cnx43, immunoreactivity at day 18 after plating. NSCs gave
rise to cardiac myocyte immunoreactive progeny. Scale bar: 150 µm.
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