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Fig. 4. Myogenic differentiation potential is correlated with insulin receptor expression. In an attempt to correlate the level of insulin signaling with myogenic differentiation potential, fluorescence activated cell sorting (FACS) was used to identify subpopulations of NSCs, based on IR expression. (A) FACS analysis showing two subpopulation of NSC based on IR expression. (B) To further correlate IR expression with myogenic potential, neurospheres were generated from EGFP-labeled IR^high and IR^low cells and allowed to expand and differentiate in MB-media. Presented is the mean±s.d. of three experiments demonstrating that myogenic potential correlates with insulin receptor expression. Note that few multilineage clones were established from IR negative founders. ^*P<0.05; ^**P<0.01. (C) IR^low NSC arise from IR^high cells during expansion. E14 IR^high cortical progenitor cells were isolated and a fraction frozen down (control) for comparison to the remainder that was expanded for 5 days, to assess the changes of IR expression during expansion. After 5 days of expansion in MB-media, the cells were resorted to examine the IR expression profile. The re-distribution of IR⁺ cells now includes IR^low-expressing and IR^neg cells. R1, IR^low population; R2, IR^high population.

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