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Fig. 6. Neural stem cells can give rise to functional cardiac myocytes. NSCs were plated at clonal density and allowed to differentiate in MB-media supplemented with 20 ng/ml bFGF on days 1 and 2 of expansion. NSC-derived cardiomyocytes were tested for functionality via mechanical coupling and responses to mediators of pace making activity. (A) Merged images of dye transfer in NSC-derived cardiomyocytes. N, neuron; S, skeletal muscle. (B) A single cardiomyocyte was preloaded with calcein-AM and diI (yellow), and parachuted over a cluster of NSC-derived cardiomyocytes. Calcein passes from the donor cell to other cardiomyocyte in the cluster. DiI, which is not transferable via gap junctions, was used to trace the location of the donor cell. (C) Presented is the beat rate of MLNSC-derived cardiomyocytes in response to sympathetic (10 µM epinephrine) and parasympathetic (2 µm acetylcholine) stimulation.





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