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Fig. 4. Rb ablation leads to hypercellular neuroendocrine lesions. Mice were mated as detailed in Fig. 1A, and pregnant dams were treated with doxycycline throughout gestation. Doxycyline was discontinued on the day of birth. (A-D) Morphological examination and immunohistochemical analysis for CGRP in adult lungs after Rb ablation (CC10-rtTA), and in control littermates lacking one or both transgenes (Control). B represents a higher magnification of the boxed area in A. Arrows mark neuroendocrine cells. Note the remainder of the airway epithelium is essentially normal. Data is representative of three CC10-rtTA mice. (E,F) Adult lung sections before (E) and after (F) microdissection of the epithelium. (G) Microdissected epithelium on thermoplastic cap used for PCR analysis. Abbreviations are as in Fig. 1. (H) PCR analysis of microdissected epithelium from double transgenic adult mice (+) with Rb^LoxP/- (top) or Rb^LoxP/LoxP (bottom) alleles, and control littermates lacking one or both transgenes (C). Representative results are shown. Note loss of the Rb^LoxP allele and the emergence of the recombined allele (Rb^Rec). L, ladder.

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