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Fig. 5. Rb family deficiency results in lung epithelial abnormalities. (A-D)
Morphological examination of transgenic (CC10-T121; A,C) and control wild-type
(WT; B,D) lungs. A pyknotic nucleus in transgenic lungs is marked with an
arrow (C). Note lack of morphologic indicators of differentiation in
transgenic lungs, including flattening of Type I cells (D, closed arrow),
ciliated cells (D, open arrow) and apical cytoplasmic protrusions indicative
of Clara cells (D, white arrowhead). Images are representative of seven
CC10-T121 founder mice. (E-H) Immunohistochemistry for Ki67 (E,F) and TUNEL
(G,H) analysis. Rare focal epithelial cells positive for Ki67 are marked with
an arrow in wild-type lungs (F). Note the majority of Ki67 immunoreactivity is
localized to mesenchymal cells in wild-type lungs (F, white arrowhead). Arrow
indicates TUNEL-positive epithelial cells in transgenic lungs (G). No specific
TUNEL staining is present in wild-type lungs (H). (I,J) Immunohistochemistry
for PCNA. Scattered focal PCNA immunoreactive epithelial (arrows) and
mesenchymal (white arrowhead) cells are indicated in wild-type lungs (J).
Ki67, TUNEL and PCNA data is representative of four CC10-T121 founder mice.
Abbreviations are as in Fig.
1.
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