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Fig. 6. Transgene expression correlates with epithelial abnormalities. (A) Western
blot analysis of whole lung lysates, showing specific expression of truncated
large T antigen (T121) in CC10-T121 transgenic (+) but not wild-type (-)
lungs. Full-length large T antigen (Lg T) or small t antigen (Sm T) is not
detected in transgenic lungs but is seen in control cell lines (C). NS,
nonspecific bands. (B-E) Immunohistochemical analysis for T121 (B,D) or TK1
(C,E) expression in transgenic lungs. Arrows indicate transgene expression in
alveolar Type II cells. (F-H) Morphological examination, Ki67 expression and
TUNEL analysis in transgenic SPC-T121 lungs. Data is representative of seven
founder CC10-T121 mice and two SPC-T121 mice. Transgene expression was
detected in two of six CC10-TK1, and seven of eight SPC-TK1 transgenic lines;
adult lungs are shown. Abbreviations are as in
Fig. 1.
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