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Fig. 6. Expression of miR164-resistant CUC2-m4 leads to
progressive boundary enlargement. Expression of erGFP under the control of
STM regulatory sequences in developing flowers following 6 days of
induction. At the end of the induction period, erGFP was expressed in a strip
two or three cells wide between the sepals of a stage 4
STM::ALCR-alcA::erGFP flower (arrow, 1). This domain was enlarged to
six to seven cells wide in a STM::ALCR-alcA::erGFP alcA::CUC2-m4
flower at similar stage (arrow, 2). Six days later, one or two cells between
the sepals expressed erGFP in a stage 6-7 STM::ALCR-alcA::erGFP
flower (arrow, 3). In STM::ALCR-alcA::erGFP alcA::CUC2-m4 flowers,
this domain was enlarged to about 10 cells (arrow, 4). In order to realise the
observations of (3) and (4), the plants had been induced again overnight to
activate erGFP expression. Scale bar: 100 µm.
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