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Fig. 3. RA signalling is required for the specification of intermediate cells. (A)
Expression of Raldh3 in the head ectoderm adjacent to the rostral
forebrain in a stage 14 chick embryo (lateral view, rostral towards the
right). The dorsal-to-ventral line indicates the level of the transversal
section shown below. Boxed area indicates intermediate telencephalic region of
the embryo. (B) Expression of transcription factors in stage 14 intermediate
explants grown alone or with BMS189453 (an antagonist of retinoid signalling
via receptors RAR
/
) (n=25). Stage 14 intermediate
explants (n=25) grown alone generated MEIS2+ cells
(22±6%) and PAX6+ cells (23±4%), but no
NKX2.1+ or EMX1+ cells were detected. In the presence of
BMS453, the generation of MEIS2+ cells was blocked and the
generation of PAX6+ cells was markedly reduced (7±3%). A
small number of NKX2.1+ cells (5±3%) were generated, but no
EMX1+ cells were detected. Scale bar: 30 µm.
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