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Fig. 5. Retinoic acid (RA) induces intermediate character in early ventral telencephalic cells. (A) A stage 8 chick embryo (dorsal view, rostral is upwards). Grey square indicates ventral telencephalic neuroectoderm explant region. (B) Expression of transcription factors in stage 8 ventral (V, st 8) explants cultured alone or with all-trans retinoic acid for 48-52 hours. Stage 8 V explants (n=15) generated NKX2.1+ cells (>90%) and ISL1+ cells (19±4%) but no MEIS2+ or PAX6+ cells. Stage 8 V explants (n=15) exposed to all-trans retinoic acid generated MEIS2+ cells (23±3%), ISL1+ cells (17±4%), PAX6+ cells (15±4%) but no NKX2.1+ or EMX1+ cells. Scale bar: 30 µm.





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