DEV01307 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 (PDF) -

  Fig. S1. Amino acid sequence comparisons among PIR and homologs from other species. The positions of stop codons and truncated proteins are indicated with arrows. The insertions in PIR relative to other homologs are labeled by an unbroken line, while the gaps are labeled with a broken line. Amino acid residues in black indicate identity; those in gray indicate conserved substitutions. GenBank Accession Numbers are: Arabidopsis thaliana, PIROGI (AY662957); Homo sapiens, HSPIR121 (NM_014376); Caenorhabditis elegans, CEGEX-2 (NP_499949); Drosophila melanogaster, DMSRA1 (AE003708); Dictyostelium discoideum, DDPIR121 (NY368270). Amino acid sequences were aligned using ClustalW using gap opening and gap extension penalties of 10 and 0.20 respectively. The alignment was refined manually to remove erroneous gaps. The sequence alignment was shaded using the boxshade software from www.embnet.org.

• Supplemental Figure 2 (PDF) -

  Fig. S2. PIROGI allele sequencing primers. Arrows indicate the forward and reverse primers that were used for PIROGI sequencing. The names and sequences of the primers.

• Supplemental Figure 3 (PDF) -

  Fig. S3. RT-PCR primers and product sizes for PIROGI, ATNAP125, DIS2, ATARPC4 and GAPC.

• Supplemental Figure 4 (PDF) -

  Fig. S4. Names and sequences of the primer pairs that were used for plasmid construction.