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Fig. 2. PIR encodes a homolog of human SRA1. Physical map of the PIR gene, mutant alleles and rescue of the mutant phenotype by overexpression. (A) The region of chromosome 5 that contains the PIR locus. Boxes indicate the position of molecular markers and the oval represents the PIR-containing BAC (T4C15). The measured recombination frequency for several markers in the mapping interval is given. (B) Physical structure of the PIR locus. The position of exons and introns are indicated by black rectangles and lines, respectively. The 5'-UTR and 3'-UTR are indicated by white rectangles. The location and nature of the pir-1, pir-2, pir-3, pir-5 and pir-T1 mutations are labeled. The location of the AT-AC intron is labeled. The 5'-UTR and 3'UTR sequences were derived from RIKEN clone RAFL04-10-J11 (GenBank Accession Numbers AV82143 and AV781909, respectively). (C) Overexpression of PIR rescues pir-3 trichome phenotype. (Left) Col mature trichome; (middle) pir-3 mature trichome; (right) mature trichome on a pir-3 plant that harbors a PIR overexpression construct.





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