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Fig. 4. Arabidopsis PIR and human SRA1 have interchangeable in vivo functions and interactions with ATNAP125. (A) Direct interaction of recombinant PIR and ATNAP125 in a GST-pull-down assay. (Top) Lanes 1-5, fractions probed with anti-ATNAP antibody; (bottom) same fractions probed with control antibody anti-AT3G. Lane 1, 5% of total binding reaction; lane 2, GST-PIR bead bound pellet fraction; lane 3, GST-PIR bead unbound; lane 4, GST bead bound; lane 5, GST bead unbound. (B)PIR and human SRA1 interact with ATNAP125 in a yeast two-hybrid assay. The bait and prey plasmids that were co-transformed into yeast are defined to the left of the corresponding yeast patches and ß-galactosidase assay results. pDS32PIR, full-length PIR; pDS32HSPIR, full-length human SRA1; pDS22ATNAP, full-length ATNAP125; pDS22HSNAP, full-length human NAP125. (C) Overexpression of human SRA1 can suppress pir-3 trichome distortion. Left to right: Col mature trichome; pir-3 mature trichome; mature trichome on a Col plant that harbors a human SRA1 overexpression construct; mature trichome on a pir-3 plant that harbors a human SRA1 overexpression construct. Scale bars: 100 µm.





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