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Fig. 2. Expression and regulation of chick Pcl2 in Hensen's node. (A-D) Whole-mount in situ hybridization analyses reveal chick Pcl2 expression in early chick embryos at stages 4 (A), 5 (B), 6 (C) and 8 (D). Asymmetric chick Pcl2 expression in the node appears from stage 5, and is restricted in the ectoderm (insert in C). (E-H) Ectopic chick Pcl2 expression is induced by Activin-A (E) and BMP4 (G) around the node, while Follistatin represses chick Pcl2 expression (F). BSA control bead does not alter chick Pcl2 expression (H). All embryos are dorsal views. Arrows indicate the node; asterisks indicate bead or bead position.





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