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Fig. 5. Knock-down of chick Pcl2 randomizes cardiac looping direction and
alters gene expression. (A-C) Ventral view of stage 11 embryo treated with
control oligonucleotide shows normal rightwards heart looping (A), while
embryo treated with antisense oligonucleotide specific to chick Pcl2,
which abolished chick Pcl2 expression as detected by whole-mount in
situ hybridization (C), exhibits reversed cardiac looping direction (B). (D,E)
Fgf8 expression is unaltered (arrows) in embryos treated with control
oligonucleotide (E), but is repressed in the right side of the node (arrows)
of embryos treated with antisense oligonucleotide to chick Pcl2 (D).
(F-M) Embryos treated with control oligonucleotide show normal expression of
Shh (F) in the node, of Caronte (H), Nodal (J) and
Pitx2 (L) in the left LPM. By contrast, embryos treated with
antisense oligonucleotide show ectopic expression of Shh in the right
side (arrow) of the node (G), and ectopic expression of Caronte (I),
Nodal (K) and Pitx2 (M) in the left LPM (arrows). Embryos in
C-M are shown dorsal views.
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