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Fig. 5. KNOX1 genes and PHAN genes are expressed in overlapping
regions in developing tomato leaves. (A) The KNOX1 gene LeT6
and LePHAN are expressed in the same domain in wild-type tomato leaf
primordia. In situ RT-PCR detection of LePHAN expression in a leaf
primordium (left) and in situ hybridization with LeT6 in a comparable
leaf primordium (right). The small arrows show expression (green fluorescence
in left, and purple in right, panel) in leaflet primordia, and the large
arrows show expression in the developing vascular trace. (B) Heterozygous
Mouse ears (Me/+) mutant tomato has increased levels of
LeT6, decreased amounts of LePHAN, and an increased number
of leaflets (KNOX1 overexpression phenotype). Homozygous
(Me/Me) mutants have even higher levels of LeT6,
causing a greater decrease in LePHAN levels. Consequently, the leaves
of these plants cannot produce blades (reduced LePHAN expression
phenotype). A was adapted, with permission, from and Kim et al. and Janssen et
al. (Kim et al., 2003; Janssen et al.,
1998). (C) The Curl (Cu) phenotype (due to
misexpression of LeT6), an antisense LePHAN phenotype (with
cup-shaped leaf), and the Cu phenotype in an antisense
LePHAN (with cup-shaped leaf) background. The misexpression phenotype
of Cu in the antisense LePHAN background is restricted to
the region of LePHAN expression.
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