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Fig. 3. Hand2 expression in Ednra–/– and
Dlx5/6–/– embryos. (A-H)
Ednra+/+;R26R;Hand2-Cre and
Ednra–/–;R26R;Hand2-Cre embryos
stained in whole mount for ß-gal activity and shown in both lateral (A,B)
and ventral views (C,D). For ventral views, the heart and outflow tract have
been removed. Embryos (not necessarily those shown in A-D) were then sectioned
along either sagittal (E,F) or frontal (G,H) planes and counterstained with
nuclear Fast Red. (A,C) In E10.5
Ednra+/+;R26R;Hand2-Cre embryos,
ß-gal-labeled cells are observed throughout pharyngeal arches 1 and 2.
(B,D) In Ednra–/–;R26R;Hand2-Cre
embryos, labeled cells are observed only along the distocaudal aspect of
mandibular arch. (E,G) In sagittal sections through the arches of
Ednra–/–;R26R;Hand2-Cre embryos,
labeled cells are confined to the neural crest-derived mesenchyme. (F,H) In
sagittal sections through the arches of
Ednra–/–;R26R;Hand2-Cre embryos,
scattered labeled cells are observed in the distal mesenchyme, with more
intense labeling observed in cells in the surrounding arch epithelium. (I-L)
Analysis of Hand2 expression in
Dlx5/Dlx6–/– embryos. Normal Hand2
expression (I,K) is absent in the pharyngeal arches of
Dlx5/Dlx6–/– embryos (J,L), although residual
expression is still observed in the distal mandibular arch (yellow arrow in
inset). A small expression domain is present in the second arch (black arrows
in L).
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