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Fig. 5. Fate of Hand2 daughter cells in
Ednra–/–;R26R;Hand2-Cre embryos.
E16.5 Ednra+/+;R26R;Hand2-Cre (A,C,E,G,I) and
Ednra–/–;R26R;Hand2-Cre (B,D,F,H,J)
embryos stained for ß-gal activity in whole-mount (A-F) or sections
(G,H), or analyzed for Hand2 expression using sectional in situ
hybridization (I,J). (A,B) In a lateral view, ß-gal-labeled cells are
observed throughout the lower jaw of
Ednra+/+;R26R;Hand2-Cre embryos (A). In
Ednra–/–;R26R;Hand2-Cre embryos,
staining is confined to ventral surface of the mandible (B). (C,D) In a
ventral view, few labeled cells are present in the lower jaw in
Ednra–/– embryos (D), although the labeling is
very prominent in the cleft between the two mandibular halves (arrow in D).
(E,F) After clearing in benzyl benzoate:benzyl alcohol, little structural
detail is apparent in the lower jaw of wild-type embryos because of the
extensive labeling (E). By contrast, labeled cells in
Ednra–/– embryos are present in the epithelial
seam between the two arch halves (arrow in F), hypoplastic tongue (t) and
lower incisors (i). (G, H) Stained sagittal cryosections illustrate labeling
in most lower jaw structures and surrounding soft tissue of wild-type embryos,
including the mandible (md), Meckel's cartilage (mc) and dental pulp of the
incisors (arrow in inset) (G). Labeling is not observed in the dental lamina
(dl; * in inset). In Ednra–/– embryos, labeled
cells are present in the dental pulp of the lower incisors (arrow in inset),
but are more scattered compared with wild-type embryos. A few labeled cells
are also present in the small residual bone and cartilage underneath the
incisors and in the dental lamina (* in inset) (H). Rugae are obvious along
the both the top and bottom of the oral cavity (arrows). (I,J) Hand2
expression is observed in the dental pulp of both wild-type and mutant
embryos, with highest expression in the odontoblast layer (yellow arrow in
insets). Expression is not observed in either embryo within the dental lamina
(black arrow in insets). p, secondary palate tissue.
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