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Fig. 1. Expression of E4BP4 during motoneuron development. (A) Whole-mount in situ hybridisation for E4BP4 on a flat-mounted E14 rat spinal cord using a mouse probe shows prominent labelling in the lumbar (L) region (arrow), weaker labelling at thoracic levels (T) and no detectable signal at sacral levels (S). (B) In situ hybridisation on sections of E9 chicken embryos using a chicken probe shows prominent labelling at limb-innervating levels (arrow). (C) Higher-magnification picture of the ventral horn of E9 lumbar spinal cord. The major motor columns defined by ISL1 labelling of an adjacent section (not shown) are indicated. MMC, medial motor column; LMCm and LMCl, medial and lateral subdivisions of the lateral motor column. (D) RT-PCR on freshly-purified E14 rat motoneurons (left panel) and chicken embryo spinal cord at indicated ages (right panel). (E) Western blot of chicken E7 spinal cord using an anti-mouse E4BP4 immune serum, performed as described (Ikushima et al., 1997).





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