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Fig. 3. E4BP4 enhances cell body growth. (A) Histograms for cell body area from a
single experiment. Motoneurons electroporated with GFP were cultured without
trophic factors (control), or with neurotrophic factor (BDNF). Motoneurons
expressing E4BP4 were cultured without BDNF. Cell body area for 80-200 neurons
in each condition was measured 1 day later and expressed in arbitrary pixel
units. There is a clear shift to larger sizes in motoneurons expressing E4BP4.
(B) Median cell body areas in each condition (mean±s.e.m.; n=5
independent experiments). Statistical significance was calculated by Student's
t-test (two-tailed). The addition of BDNF to E4BP4 electroporated
motoneurons did not further increase cell body size (7.3±0.3 pixels;
n=3).
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