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Fig. 4. E4BP4 potently stimulates axonal outgrowth. (A) Typical images of control
and E4BP4-expressing motoneurons after 1 day in culture, as used for
subsequent image analysis. Scale bar: 20 µm. (B) Cumulative plot of length
of longest neurite from one representative experiment. For each value of
neurite length, the percentage of the total number of neurons having a neurite
at least that length is plotted. A shift of the curve to the right thus
indicates increased axonal growth. Values from control neurons (black line),
control neurons with BDNF (broken line) and E4BP4-expressing neurons without
BDNF (grey line) are shown. (C) Median values for length of the longest
neurite, expressed relative to control neurons with BDNF, fixed as 1
(mean±s.e.m.; n=5 independent experiments).
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