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Fig. 4. Localization of E-cadherin and ß-catenin in embryos lacking the
binding partner. (A) E-cadherin is detectable on the blastomere surface of
embryos expressing truncated ß-catenin. E-cadherin is visible on the
surface of the blastomeres of both the control embryos (+/+) and the embryos
expressing truncated ß-catenin (Cre/Ø). E-cadherin is also
detectable in the cytoplasm of 2-cell and 4-cell stage embryos expressing
truncated ß-catenin. (B) ß-catenin is present in the nucleus of
early embryos lacking maternal E-cadherin. ß-catenin was detected in
control embryos (+/+) and embryos lacking maternal E-cadherin (Cre/Ø),
using the polyclonal ß-catenin antibody recognizing the C terminus of the
protein. ß-catenin is visible on the blastomere surface of control
zygotes, 2-cell stage embryos and 8-cell stage embryos, with some
ß-catenin also visible in the cytoplasm of control zygotes and 2-cell
stage embryos. By contrast, ß-catenin is visible in the pronuclei and
cytoplasm of zygotes, as well as in the nuclei and cytoplasm of 2-cell stage
embryos lacking maternal E-cadherin. Only a small amount of ß-catenin is
detectable in 8-cell embryos lacking maternal E-cadherin. (C) Truncated
ß-catenin is present in the nucleus of embryos lacking maternal
E-cadherin. ß-catenin was detected in control embryos (+/+), and in
embryos expressing truncated ß-catenin and lacking maternal E-cadherin
(double mutant) embryos (Cre/Ø), using the polyclonal ß-catenin
antibody that detects the C-terminal part of the protein. ß-catenin is
detected in the pronuclei of control and double-mutant zygotes, on the surface
and in the cytoplasm of control 2-cell stage embryos, in the nuclei and
cytoplasm of double-mutant 2-cell stage embryos, and on the surface of 4 to
8-cell stage control and double-mutant embryos. Note for B and C, blastomeres
of 8-cell stage embryos lacking maternal E-cadherin do not adhere to each
other. Consequently all manipulations during the immunostaining process were
carried out with extreme caution to maintain blastomeres of embryos in a
clump. No fluorescence was detected in embryos where the primary antibody was
omitted (data not shown). Scale bars: 10 µm.
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