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Fig. 5. The level of ß-catenin in 2-cell stage embryos is controlled by the
same mechanism as in somatic cells. (A) Involvement of the proteasome. The
presence (+) or absence (–) of maternal E-cadherin
(E-cadherinmat) in the embryos analyzed is indicated, as is the
presence (+) or absence (–) of the proteasome inhibitor MG132 (5 µM).
The monoclonal ß-catenin antibody (ß-catenin Ab) was used for
staining (+), except where it was omitted (–) to determine background
levels of staining. Secondary antibody was used in every staining. Scale bar:
10 µm. (B) Transcripts for proteins interacting with ß-catenin in the
cytoplasm or nucleus are present in oocytes and early embryos. Names of the
transcripts are indicated to the left of the figure.
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