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Fig. 6. (A) Embryos expressing truncated ß-catenin do not develop optimally,
as indicated by fewer pups per litter. Bar graph depicting the mean number of
pups per litter obtained from females producing oocytes expressing truncated
ß-catenin
(ßF/ßF;cre/Ø) and
control females (ßF/ßF and
ßF/ß;cre/Ø). The number of litters
recorded (n) in each group is indicated at the bottom of the graph.
(B,C) The paternal alleles of ß-catenin and E-cadherin are activated
sequentially. (B) Expression of E-cadherin in embryos lacking maternal
E-cadherin (top) and control embryos (bottom). (C) Expression of wild-type
ß-catenin in embryos expressing truncated ß-catenin (top) and
control embryos (bottom). The expression of both genes was monitored by RT-PCR
and subsequent Southern blot analysis of the PCR products using a
32P-labeled E-cadherin cDNA probe. Wild-type ß-catenin was
monitored using primers situated in sequences coding for the N-terminal part
of the protein. The gene product monitored is indicated on top of the figure;
the genotype of the females from which the embryos were isolated is indicated
on the right. Mitochondrial ATP synthase (mt-Atp6) was used as a
control for the RT-PCR, and was detected by ethidium bromide staining. Embryo
stages are the same as in Fig.
2.
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