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Fig. 3. Rapid activation of RhoB after ectopic LSox5 expression in the cephalic neural tube. Expression of EGFP (A); LSox5 (B,E,F) or RhoB (C,D,F) in embryos fixed 6-7 hours after electroporation with pCX-LSox5. A few transformed cells ectopically expressing LSox5 can be detected scattered within the neural tube with the anti-LSox5 serum (A,B). In situ hybridisation for RhoB at a mesencephalic level shows that the ectopic activation of this gene in cells at the electroporated side is analogous to the expression of LSox5 (C). The cells that ectopically express RhoB correspond to those with higher levels of ectopic LSox5 expression in their nuclei (D-F).





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