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Fig. 4. Dgo, with Pk and Stbm, promotes the apical localization of Fmi and Fz.
Apical confocal sections are shown; anterior is leftwards and the equator at
bottom. MF is at left margin of panels, except in F where it is indicated by
red arrowhead. Mutant tissue is marked by absence of ß-gal staining or
GFP (blue). White/yellow arrows indicate PCP protein localization in R3/R4
pair (
row 3 or 4) and arrowheads indicate the R4-like enrichment (row 5
and posterior), except in F where it indicates clones ahead of MF. Fmi (green)
and Fz-GFP (red) localization is analyzed in mutant tissue of null alleles of
the respective PCP genes. (A) pk–: no significant
abnormalities in the apical localization of Fmi. (B) pk–,
dgo–: loss of apical Fmi localization. (C)
pk–, dgo–: reduction in apical Fz
localization. (D) dgo–, stbm–: loss
of apical Fmi localization (compare with A and B). (E)
dgo–, stbm–: loss of apical Fz-GFP
localization. The vertical arrow indicates a mosaic in which the R3 specific
Fz-GFP staining is missing. (F) pk–,
stbm– mutant clones behind and ahead of MF (marked by
red arrowhead): apical Fmi localization is lost in mutant tissue ahead of MF,
similar to pk–, dgo– and
pk–, stbm– double mutants (compare
with B and C), but apical localization of Fmi is observed posterior to MF,
although no clear pattern is detected. In pk–,
stbm– double mutant tissue there is a different effect
on Fmi anterior and posterior to MF. (F) pk–,
stbm–: apical Fz-GFP localization behaves like Fmi: it
is unaffected posterior to MF (but lost anterior to MF, not shown).
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