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Fig. 6. Dgo physically interacts with Pk and Stbm in vitro. (A) Dgo Ankyrin repeats bind to a 131 amino acid fragment of Pk (white box in scheme). GST fusion proteins with fragments of the C terminus of Pk indicated at the top (color coded below, compare with scheme) were tested for binding to full-length Dgo (³⁵S-Dgo in vitro translated; marked yellow in the scheme on the right) or to its Ankyrin repeat region (³⁵S-Dgo Ank). An unrelated control (³⁵S-Control) does not bind to Pk. As a standard, 10% of the in vitro translated protein used for the binding reaction was loaded directly (10% input). The Pk region interacting with Dgo comprises residues 820-908. Purple boxes in Dgo scheme on right indicate the Ankyrin repeats and light and dark green boxes in scheme underneath indicate the PET and the three LIM domains of Pk, respectively. (B) Dgo Ankyrin repeats interact with Stbm. In vitro translated fragments of the C-terminal cytoplasmic region of Stbm (indicated on left and marked in yellow in scheme on right) were tested for their binding to a GST fusion protein of the Ankyrin repeats of Dgo (G-Dgo Ank) or to an unrelated fusion protein (G-Control). ³⁵S-Stbm CtermN2 and ³⁵S-Stbm CtermC2 define a region of 85 amino acids required for binding of Stbm to Dgo. Standard is as in A. Light green boxes in scheme on right indicate position of the predicted four transmembrane domains of Stbm.

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