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Fig. 7. Cell proliferation and expression of cyclin-dependent kinase inhibitors in
Foxp1–/– hearts. Immunohistochemistry was
performed on wild-type (A,C,E,G) and Foxp1–/–
(B,D,F,H) hearts at E13.5 using antibodies for phospho-histone H3 (A,B), p21
(C,D), p27 (E,F), and p57 (G,H). Significant increases in phospho-histone H3
(B, red arrowheads) and p21 staining (D, red arrowheads) were observed, while
a decrease in p27 expression was observed in the compact zone of
Foxp1–/– hearts (F, red arrow). Quantifying
the number of phospho-histone H3 positive cells in the trabecular zone for
wild-type and Foxp1–/– hearts shows an
approximately threefold increase in the number of mitotic cells in the
trabecular zone of Foxp1–/– hearts but no
change in the compact zone (I). Identical analysis of p21 expression levels
reveals an approximate fourfold increase in trabecular myocardium but no
significant difference in compact myocardium (J). p27 levels were decreased by
almost 50% in Foxp1–/– compact myocardium,
while levels in trabecular myocardium were unchanged (K). Scale bar: 200
µm.
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