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Fig. 8. Myocardial differentiation is disrupted in
Foxp1–/– hearts. In-situ hybridization was
performed using probes specific for Irx3 (A,B), Anf (C,D)
and N-myc (E-H) on wild-type (A,C,E,G) and
Foxp1–/– (B,D,F,H) hearts at E11.5 (E,F) and
E14.5 (A-D,G,H). Irx3 expression is expanded to encompass almost all
compact and trabecular myocardium (B). N-myc expression was
significantly reduced in the ventricular wall of
Foxp1–/– embryos (compare E,F,G,H). H+E
staining of wild-type and Foxp1–/– hearts at
E11.5 shows a thin compact zone in the myocardial wall of the ventricle wall
of Foxp1–/– hearts (I,J, brackets).
Transmission electron microscopy was performed on E11.5 wild-type (K) and
Foxp1–/– (L) hearts. Wild-type compact zone
myocardium shows a laminated appearance, while
Foxp1–/– heart compact zone myocardium has a
disorganized appearance (compare bracketed regions). Scale bars: 250 µm in
A,B; 400 µm in C-F; 800 µm in G,H; 100 µm in I,J.
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