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Fig. 4. kette mutants stop fusion during formation of electron-dense
plaques. (A-C) Transmission electron microscope analyses of
ketteJ4-48, blow2 and mbc
mutants confirm that ketteJ4-48 and
blow2 mutants do form muscle precursor cells, while
mbc mutants do not. Scale bars: 2 µm. (A) Stage 14
ketteJ4-48 mutant embryo; asterisks indicate precursors
with two or three nuclei. (B) Stage 12-13 blow2 mutant
embryo; developing precursors with two nuclei are clearly visible. (C) Stage
13-14 mbc mutant embryo. (D-F) Stage 13 wild-type embryo. (D) A
muscle precursor has established contact with fusion-competent myoblasts,
while groups of electron dense vesicles start to build the prefusion complex
of paired vesicles (arrowheads). Nearby, a prefusion complex has already
started to dissolve and will form a electron-dense plaque (arrow). Scale bar:
1.5 µm. (E,F) Detailed view of a group of electron dense vesicles in D. (F)
Dissolving prefusion complex and developing electron dense-plaque (arrow)
forming within a cloud of vesicles. Scale bar: 500 nm. (G) Developing
electron-dense plaque in a stage 15 ketteJ4-48 embryo.
Remains of the dissolving prefusion complex are still visible (arrow); the
length of the plaque is nearly twice that of the wild type plaque described by
Doberstein et al. (Doberstein et al.,
1997). Scale bar: 150 nm.
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