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Fig. 5. Kette and Blown fuse interact genetically. (A-D) Anti-ß3-Tubulin fluorescent staining of stage 15 embryos. (A) Homozygous kette^G1-37 hypomorphic mutant. (B) Homozygous blow² null mutant. (C) Homozygous kette^G1-37 mutant with only one intact copy of blow (an enhancement of fusion defect takes place). (D) Double homozygous blow² and kette^G1-37 mutant (further enhancement of fusion defects leads to a phenotype that resembles the original blow² phenotype more than the kette^G1-37 phenotype). (E,F) Anti-ß3-Tubulin fluorescent staining (red); anti-ß-galactosidase staining (green) of rP298-lacZ enhancer trap. (E) Stage 16 blow² mutant embryo (only minimuscles, presumably representing precursors, are formed, lareg gaps in the somatic mesoderm allow a direct view of the gut); ß3-Tubulin-positive cardioblasts are properly arranged. At this stage, unfused myoblasts have been engulfed by macrophages. (F) Mesodermal overexpression of UAS-hem with a twist-GAL4 driver line in blow² mutant background rescues the blow phenotype, at least partially, at stage 16. The embryo forms clearly fused and attached muscles, although defects in muscle number, size and attachment occur.

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