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Fig. 4. vhnf1 and val mutant cells behave equivalently in genetic
mosaics. (A) Donor-derived cells (brown) from a wild-type embryo contribute
throughout the hindbrain of a wild-type host embryo. Krox20
expression in r3 and r5 is in blue. (B) vhnf1– cells
are excluded from r5 and r6 of wild-type host embryos. (C) Wild-type cells
form tight aggregates (arrowheads) in the r5-6 region of
vhnf1– host embryos. These wild-type-derived cells
express krox20 when in the r5 region. (D)
vhnf1– cells were able to contribute throughout the
hindbrain of val– host embryos, including the
presumptive r5-6 region (bracket), and conversely (E)
val– cells contributed to the entire hindbrain of
vhnf1– host embryos. Cell behaviors in genetic
mosaics are consistent with similar changes in Eph and
ephrin expression in val and vhnf1 mutant embryos.
(F,I) Wild-type (wt); (G,J) val–; (H,K)
vhnf1–. (F-H) At 11.6 hpf, r5 and r6 expression of
EphB4a (blue) is normally expressed in r2, r3, r5 and r6 of the
hindbrain (F). The expression in r5 and r6 is strongly reduced in both
val– (G) and vhnf1– (H)
embryos. (I-K) ephrin-B2a (blue) is normally expressed in r1, r4 and
r7 at 12 hpf (I). Its expression is upregulated posterior to r4 in
val– (J) and vhnf1– (K)
embryos. krox20 expression in r3 and r5 is in purple (F-H) or red
(I-K). Embryos are shown in dorsal views with anterior to the left.
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