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Fig. 3. Tissue-specific repression of glide complex activity affects muscle organisation and locomotion. (A) Locomotion assay. Statistic evaluation of second instar larvae motility: wild-type (WT), ptc::glideN7-4DN (glideN7-4DN) and ptc::glideDN (glideDN) (n=6). Error bars indicate s.e.m.; statistical significance was calculated using Student's t-test. Significant differences versus wild-type are indicated on top of bar (***P<0.0001). Control (glideN7-4DN) and wild-type (WT) larvae do not show any difference, whereas ptc::glideDN larvae (glideDN) display reduced motility. (B-E) Polarised light micrographs showing muscle organisation in ptc::glideN7-4DN (B,D) and ptc::glideDN (C,E) second instar larvae. Dorsal to the top. Muscle attachment sites are severely altered in ptc::glideDN larvae. (D,E) Regions delimited by dashed boxes in B,C, respectively. (F-K) Muscle F-actin organisation (visualised by phalloidin labelling) on ptc::glideN7-4DN (F-H) and ptc::glideDN (I-K) late-stage 17 embryos. Dorsal to the top. (G,J) show the regions delimited by dashed boxes in F,I, respectively. Lateral (F,G,I,J) or dorsal views (H,K). Dorsal midline is indicated by dashed line in (H,K). Scale bars: 1 mm in B,C; 0.1 mm in D,E; 50 µm in F-K.





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