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Fig. 6. Epistatic relationships between the glide complex and stripe reveal a novel molecular tendon cell-specific pathway. (A-F) Anti-Stripe or anti-Alien immunolabelling on wild-type (A,D, respectively) and on either en::glide (B,E, respectively) or en::glide2 (C,F, respectively) stage-15 embryos. Dorsal views, dorsal midline is represented by a dotted line. (B) stripe ectopic expression is indicated by arrowhead. Note that ectopic glide2 does not induce stripe expression (C). (D-F) Alien ectopic expression induced by either glide (E) or glide2 (F) is indicated by arrowheads. (G-I) Ent2 in-situ hybridisation on wild-type (G), en::glide (H, glide gof) and en::stripe b (I, stripe b gof) stage-14 embryos. Lateral views, dorsal to the top. Ent2 is expressed in segment border cells (bracket in G). Note that glide, but not stripe b, ectopic expression induces Ent2 expression (compare brackets in H,G and I,G, respectively). Scale bars: 50 µm.

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