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Fig. 3. lbl1 suppresses the epidermal patterning defects in
Rld1-O. Scanning electron micrographs of the adaxial (A1-E1) and
abaxial (A2-E2) epidermal surfaces of adjacent mature adult leaves samples.
(A3-E3) High magnification of selected images to illustrate specific epidermal
patterning defects. Compared with wild-type leaves (A), Rld1-O leaves
(B) display normal polarity near the margins, but, in the center of the
lamina, bulliform cells and macrohairs develop on the abaxial surface. Note
the presence of isolated macrohairs (arrow in B3) and the overlap between
macrohairs on the adaxial and abaxial epidermis. The regular spacing between
bulliform cell files and between macrohairs is disrupted in weakly phenotypic
lbl1-ref leaves (C), and bulliform cell files are frequently
disrupted (arrow in C3). lbl1-ref Rld1-O leaves with a mild rolled
phenotype (D) develop bulliform cells on both the adaxial and abaxial
epidermis, and macrohairs on the abaxial epidermis in some regions of the
blade. As in lbl1-ref, bulliform cell files are discontinuous and
irregularly spaced (arrow in D3). Epidermal patterning in lbl1-ref
Rld1-O leaves with a flattened morphology (E) is indistinguishable from
that of wild type. ad, adaxial; ab, abaxial; mh, macrohair; bc, bulliform
cell. All samples are oriented with the margin towards the right. Scale bars:
in A1-E2, 1 mm; in A3-E3, 0.25 mm.
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