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Fig. 4. lbl1 acts upstream of rld1. In situ hybridization shows
that rld1 expression in wild type (A,B) occurs in the SAM and
vasculature, and on the adaxial side of leaf primordia. In lbl1
apices (C,D), rld1 expression persists in the vasculature but is
variably reduced in the SAM and on the adaxial side of developing primordia.
Arrows in A and B mark selected regions expressing rld1; arrows in C
and D mark the absence of rld1 transcripts in the corresponding
positions. (A,C) Longitudinal sections; (B,D) transverse sections. RT-PCR
analysis (E) on vegetative apices and young leaf primordia from wild type,
lbl1-rgd1, Rld1-O and the lbl1 Rld1-O double mutant
indicates that rld1 transcript levels are reduced in
lbl1-rgd1 but increased in Rld1-O. In the double mutant,
rld1 transcripts accumulate to a level intermediate between that of
either single mutant. Ubiquitin (ubi) transcripts were
amplified as a control. A, apices comprising the meristem and approximately
four young leaf primordia; LP, P5-P8 leaf primordia.
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