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Fig. 8. The yabby genes act downstream of lbl1 and rld1.
Relative to wild type (A), zyb14 expression is reduced in
lbl1-rgd1 (B,C). In the narrow lbl1 leaf primordia,
zyb14 is absent from mutant margins (arrows in C), but remains
localized to the adaxial side of normal margins. Expression of zyb14
is induced uniformly in ectopic lamina on the adaxial surface of lbl1
leaf primordia (arrow in D). zyb14 is expressed more abundantly in
Rld1-O (E), but the pattern of expression in young Rld1-O
primordia resembles that observed in wild type. Ectopic outgrowths arise on
the abaxial surface of Rld1-O leaf primordia at the boundary of
sectors expressing zyb14 (F) or zyb9 (G), and sectors that
lack yabby expression (e.g. asterisk in F). These abaxial ectopic
outgrowths also induce both zyb14 and zyb9 expression
(arrows in F and G, respectively). RT-PCR analysis on vegetative apices and
young leaf primordia from wild type, lbl1-rgd1, and Rld1-O
in the A158 inbred indicates that the relative expression levels of
zyb14 (H) and zyb9 (I) are reduced in lbl1-rgd1
apices, and slightly increased in Rld1-O. ubi transcripts were
amplified as a control. A, apices comprising the meristem and approximately
four young leaf primordia; LP, P5-P8 leaf primordia.
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