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Fig. 1. Maternal N14 mutant embryos have a defect in pole cell maintenance. Wild-type embryos (A,C,E,G) and N14^m– embryos from N14/Df(2R)w45-19g females (B,D,F,H) were stained for Vasa to visualize pole cells. Anterior is towards the left in all panels. (A-F) Lateral views. (G,H) Top views. In wild-type embryos, pole cells were formed at the posterior pole of the blastoderm-stage embryo (A), and carried into the posterior midgut primordium. At stage 10, pole cells moved through the midgut epithelium and migrated dorsally along its basal surface (C). Next, pole cells moved into the mesoderm at stage 11 (E), and were finally incorporated into the embryonic gonad at stage 16 (G). In N14^m– embryos, a normal number of pole cells was formed (B). These pole cells migrated normally through the midgut epithelium and moved dorsally along its surface (D). However, the number of Vasa-positive pole cells was rapidly reduced at stage 11 (F), and few or no pole cells were incorporated into the gonad (H).

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