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Fig. 3. Pole cell death in N14m– embryos might occur via
a caspase 3-independent pathway. Pole cells in N14m–
embryos were directly labeled using a caged fluorescein (A, green), and were
stained for cleaved caspase 3 (B, red) and Vasa (C, blue). (D) Merged image of
A-C. Cleaved caspase 3 was never detected in the dying pole cells on the
surface of the midgut, in which the fluorescein signal remained but the Vasa
signal was reduced (arrowheads). Arrows indicate the active caspase 3-positive
mis-migrated pole cells, which are usually seen even in wild-type embryos.
Scale bar: 40 µm.
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