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Fig. 5. N14 mutation does not affect zygotic gene expression in pole
cells. (A,B) Embryos at the syncytial blastoderm stage were stained with the
antibody H5 (green) and an antibody against Vasa (red). The H5 antibody
labeled somatic nuclei, but not pole cell nuclei in
N14m– embryos (B) as well as in wild-type embryos
(A). Scale bar: 20 µm. (C-E) Expression of ß-galactosidase in pole
cells was examined in embryos from nanos-Gal4-VP16 females crossed
with UAS-lacZ males. (C,D) Lateral views of stage 10 embryos. In both
N14m– (D) and wild-type (C) embryos,
ß-galactosidase was expressed in pole cells. (E) The stage-dependent
expression of ß-galactosidase was examined in
N14m– background (black squares) and wild-type
background (white squares). The percentage of embryos with
ß-galactosidase-positive pole cells was plotted against the embryonic
stage. In both N14m– and wild-type background, the
expression of zygotic ß-galactosidase was detected from stage 9. After
stage 12, the number of embryos expressing ß-galactosidase was diminished
in N14m– embryos, owing to pole cell loss.
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