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Fig. 1. (A) Western transfer analysis of transformant clones generated using the
gskA disruption construct. Axenically growing cells were harvested
and GskA protein was analysed as described in methods. Protein loading and
transfer onto the membrane was normalised by staining with Ponceau S. (B) GSK3
kinase activity measured in cell extracts from AX2 cells and
gskA-null clones. Growing cells were harvested and GSK3 levels
assayed as described (Ryves and Harwood, 1998). Each sample was assayed in
triplicate and means and standard deviations are shown.
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