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Fig. 3. (A) Development on non-nutrient agar of Ax2/gskA cells pregrown in
either axenic medium or bacteria. Cells of a random integrant control were
compared with an Ax2/gskA strain, grown either axenically or in the
presence of a bacterial food source. They were then allowed to develop on
KK2-agar and photographed from above. (B) Slug migration on water
agar. Cells from a random integrant clone and an Ax2/gskA strain were
grown in axenic medium, developed to the slug stage and allowed to migrate on
water agar for 24 hours and towards a unidirectional light source (indicated
by arrows). Approximately 80% of the gskA-null structures developed
to the slug stage. The structures and their associated slime trails were
transferred onto a transparent membrane and visualised by protein
staining.